Anti-Ricin [F8(JOB10)]

Absolute Antibody

Anti-Ricin [F8(JOB10)]

9

The antibody is specific for RTA. The antibodys principal contacts with RTA were centered on beta-strand h, with lesser interactions occurring with alpha-helix B and alpha-helix D. Ricin is a member of the type II ribosome-inactivating protein (RIP) famil

The crystal structure of the VHH antibody in complex with the ricin toxins enzymatic subunit (RTA) was solved. The specificity of the original format of the antibody was confirmed by ELISA analysis, and the antibodys EC50 for ricin holotoxin was 0.5-0.8 nM. The binding affinity of the VHH fragments to ricin was measured by surface plasmon resonance (Kd= 0.2 nM). In the Vero cell cytotoxicity assay, the VHH had an IC50 of ~300 nM, thereby classifying it as a weak neutralizing antibody (Rudolph et al., 2016; PMID: 27159829).

ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

F8(JOB10)

LOC8261245

ricin

proricin A chain; RCOM_2159810; ricin; ricin E beta chain; ricin homolog, truncated

Rabbit

IgG

Ricin

Plant

-20°C,2°C to 8°C

12352203