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Immunohistochemical staining of human cerebellum shows moderate positivity in neuronal processes in molecular layer.
Immunohistochemical staining of human cerebellum shows moderate positivity in neuronal processes in molecular layer.
Immunohistochemical staining of human cerebellum shows moderate positivity in neuronal processes in molecular layer.

Anti-RIT2 Antibody

HPA072174
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRIT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RIT2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6014
  • Target name
    RIT2
  • Target description
    Ras like without CAAX 2
  • Target synonyms
    GTP-binding protein Rit2; GTP-binding protein Roc2; ras-like protein expressed in neurons; ras-like without CAAX protein 2; RIBA; Ric-like, expressed in neurons; RIN; ROC2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99578
  • Protein Name
    GTP-binding protein Rit2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RIT2 using anti-RIT2 antibody (A07833-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SK-N-SH whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIT2 antigen affinity purified polyclonal antibody (Catalog # A07833-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIT2 at approximately 17,21 kDa. The expected band size for RIT2 is at 25 kDa.
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