Anti-RNA hairpin [BL3-6S97N]

Absolute Antibody

Anti-RNA hairpin [BL3-6S97N]

9

The antibody binds to the RNA hairpins GAAACAC hairpin. An RNA hairpin is an essential secondary structure of RNA. It can guide RNA folding, determine interactions in a ribozyme, protect messenger RNA (mRNA) from degradation, serve as a recognition motif

The Fab was generated by directed mutagenesis from the parental Fab BL3-6. The S97N mutation in the complementarity determining region L3 of the Fab improved the binding affinity compared to the parent Fab BL3-6 (Kd = 5 nM). The Fab fragment was also found to bind the affinity-matured GAGACCC hairpin. The crystal structure of the Spinach RNA grafted with GAAACAC loop in complex with Fab BL3-6S97N was determined (Koirala et al., 2018; PMID: 29309709).

Other Application

Research Use Only

Monoclonal

BL3-6S97N

Human

Other Species

-20°C,2°C to 8°C

12352203