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Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, actin filaments & vesicles.
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, actin filaments & vesicles.
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane, actin filaments & vesicles.

Anti-RND1 Antibody

HPA077800
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRND1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RND1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27289
  • Target name
    RND1
  • Target description
    Rho family GTPase 1
  • Target synonyms
    ARHS, RHO6, RHOS, rho-related GTP-binding protein Rho6, ras homolog gene family, member S
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92730
  • Protein Name
    Rho-related GTP-binding protein Rho6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RND1 using anti-RND1 antibody (A06347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RND1 antigen affinity purified polyclonal antibody (Catalog # A06347) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RND1 at approximately 26KD. The expected band size for RND1 is at 26KD.
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IHC image of CSB-PA835694LA01HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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