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Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.

Anti-RNF111 Antibody

HPA038577
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetRNF111
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNF111 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54778
  • Target name
    RNF111
  • Target description
    ring finger protein 111
  • Target synonyms
    ARK, hRNF111, E3 ubiquitin-protein ligase Arkadia, Arkadia, RING-type E3 ubiquitin transferase Arkadia
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZNA4
  • Protein Name
    E3 ubiquitin-protein ligase Arkadia
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RNF111 using anti-RNF111 antibody (A05708-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF111 antigen affinity purified polyclonal antibody (Catalog # A05708-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF111 at approximately 109 kDa. The expected band size for RNF111 is at 109 kDa.
Product group Antibodies
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Anti-RNF111 Antibody Picoband(r)A05708-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRNF111
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Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF111 Polyclonal Antibody, Unconjugated (bs-10937R) at 1:200 overnight at 4°C, DAB staining.
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RNF111 Polyclonal AntibodyBS-10937R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetRNF111
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Atlas Antibodies
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Protein IDQ6ZNA4
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Product group Antibodies
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TargetRNF111
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Product group Antibodies
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RNF111 AntibodyCSB-PA030146
ApplicationsWestern Blot, ELISA
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TargetRNF111
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