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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.

Anti-RNF44 Antibody

HPA038981
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRNF44
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNF44 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22838
  • Target name
    RNF44
  • Target description
    ring finger protein 44
  • Target synonyms
    RING finger protein 44
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L0R7
  • Protein Name
    RING finger protein 44
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RNF44 using anti-RNF44 antibody (A16090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF44 antigen affinity purified polyclonal antibody (Catalog # A16090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF44 at approximately 48 kDa. The expected band size for RNF44 is at 48 kDa.
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