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Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Anti-RNPEP Antibody

HPA061487
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetRNPEP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNPEP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6051
  • Target name
    RNPEP
  • Target description
    arginyl aminopeptidase
  • Target synonyms
    aminopeptidase B; APB; AP-B; arginine aminopeptidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H4A4
  • Protein Name
    Aminopeptidase B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AP-B/RNPEP using anti-AP-B/RNPEP antibody (A10683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP-B/RNPEP antigen affinity purified polyclonal antibody (Catalog # A10683-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP-B/RNPEP at approximately 73 kDa. The expected band size for AP-B/RNPEP is at 73 kDa.
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