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Immunohistochemical staining of human retina shows strong cytoplasmic positivity in rod outer segments.
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in rod outer segments.
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in rod outer segments.

Anti-ROM1 Antibody

HPA073279
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetROM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ROM1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6094
  • Target name
    ROM1
  • Target description
    retinal outer segment membrane protein 1
  • Target synonyms
    ROM, ROSP1, RP7, TSPAN23, rod outer segment membrane protein 1, tetraspanin-23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03395
  • Protein Name
    Rod outer segment membrane protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ROM1 using anti-ROM1 antibody (A07035-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROM1 antigen affinity purified polyclonal antibody (Catalog # A07035-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROM1 at approximately 37 kDa. The expected band size for ROM1 is at 37 kDa.
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