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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & plasma membrane.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & plasma membrane.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & plasma membrane.

Anti-RP2 Antibody

HPA000909
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RP2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6102
  • Target name
    RP2
  • Target description
    RP2 activator of ARL3 GTPase
  • Target synonyms
    DELXp11.3, NM23-H10, NME10, TBCCD2, XRP2, protein XRP2, RP2, ARL3 GTPase activating protein, retinitis pigmentosa 2 (X-linked recessive)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75695
  • Protein Name
    Protein XRP2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Western blot All lanes: RP2antibody at 3.81ug/ml Lane 1: Jurkat whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
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Figure 1. Western blot analysis of RP2 using anti-RP2 antibody (A01923-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SGC-7901 whole cell lysate, Lane 3: rat lung tissue lysates, Lane 4: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RP2 antigen affinity purified polyclonal antibody (Catalog # A01923-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.
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