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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and glial cells.

Anti-RPL29 Antibody

HPA069064
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPL29
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPL29 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6159
  • Target name
    RPL29
  • Target description
    ribosomal protein L29
  • Target synonyms
    HIP, HUMRPL29, L29, RPL29P10, RPL29_3_370, eL29, large ribosomal subunit protein eL29, 60S ribosomal protein L29, HP/HS-interacting protein, cell surface heparin-binding protein HIP, heparin/heparan sulfate-binding protein, heparin/heparan sulfate-interacting protein, ribosomal protein YL43 homologue
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP47914
  • Protein Name
    Large ribosomal subunit protein eL29
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RPL29 using anti-RPL29 antibody (A05949-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL29 antigen affinity purified polyclonal antibody (Catalog # A05949-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL29 at approximately 20 kDa. The expected band size for RPL29 is at 20 kDa.
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