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Immunohistochemical staining of human epididymis shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human epididymis shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human epididymis shows strong granular cytoplasmic positivity in glandular cells.

Anti-RPN2 Antibody

HPA008297
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPN2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6185
  • Target name
    RPN2
  • Target description
    ribophorin II
  • Target synonyms
    RIBIIR, RPN-II, RPNII, SWP1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, oligosaccharyltransferase complex subunit (non-catalytic), ribophorin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04844
  • Protein Name
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RPN2 using anti-RPN2 antibody (A05006-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN2 antigen affinity purified polyclonal antibody (Catalog # A05006-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN2 at approximately 69 kDa. The expected band size for RPN2 is at 69 kDa.
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