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ProductsBack/Anti-RPRD1A [RAB-C113]

Anti-RPRD1A [RAB-C113]

Ab01855-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
Product group Antibodies
TargetRPRD1A
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-RPRD1A [RAB-C113]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP; IP-MS; IF.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
  • Applications Supplier
    FC; spiked-IP; IP-MS; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C113
  • Gene ID55197
  • Target name
    RPRD1A
  • Target description
    regulation of nuclear pre-mRNA domain containing 1A
  • Target synonyms
    HsT3101, P15RS, regulation of nuclear pre-mRNA domain-containing protein 1A, CDKN2B-related protein, Cyclin-dependent kinase inhibitor 2B-related protein (p15INK4B-related protein), cyclin-dependent kinase 2B-inhibitor-related protein, p15INK4B-related protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96P16
  • Protein Name
    Regulation of nuclear pre-mRNA domain-containing protein 1A
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing RPRD1A protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P15RS/RPRD1A antigen affinity purified polyclonal antibody (Catalog # A10062-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P15RS/RPRD1A at approximately 36 kDa. The expected band size for P15RS/RPRD1A is at 36 kDa.
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