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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-RPRD1B Antibody

HPA070590
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPRD1B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPRD1B Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID58490
  • Target name
    RPRD1B
  • Target description
    regulation of nuclear pre-mRNA domain containing 1B
  • Target synonyms
    C20orf77, CREPT, K-H, Kub5-Hera, NET60, dJ1057B20.2, regulation of nuclear pre-mRNA domain-containing protein 1B, Ku70-binding protein 5-Hera, cell-cycle related and expression-elevated protein in tumor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQG5
  • Protein Name
    Regulation of nuclear pre-mRNA domain-containing protein 1B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RPRD1B using anti-RPRD1B antibody (A07903-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPRD1B antigen affinity purified polyclonal antibody (Catalog # A07903-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPRD1B at approximately 37 kDa. The expected band size for RPRD1B is at 37 kDa.
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