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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-RPS15A Antibody

HPA047103
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRPS15A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPS15A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6210
  • Target name
    RPS15A
  • Target description
    ribosomal protein S15a
  • Target synonyms
    40S ribosomal protein S15a; DBA20; S15a; small ribosomal subunit protein uS8; up-regulated by HBV X protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62244
  • Protein Name
    40S ribosomal protein S15a
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPS15A using anti-RPS15A antibody (A09164-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS15A antigen affinity purified polyclonal antibody (Catalog # A09164-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS15A at approximately 15 kDa. The expected band size for RPS15A is at 15 kDa.
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