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Immunohistochemical staining of human cervix, uterine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cervix, uterine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cervix, uterine shows strong cytoplasmic positivity in glandular cells.

Anti-RPS25 Antibody

HPA031801
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPS25
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPS25 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6230
  • Target name
    RPS25
  • Target description
    ribosomal protein S25
  • Target synonyms
    S25, eS25, small ribosomal subunit protein eS25, 40S ribosomal protein S25
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62851
  • Protein Name
    Small ribosomal subunit protein eS25
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPS25 using anti-RPS25 antibody (A09150-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS25 antigen affinity purified polyclonal antibody (Catalog # A09150-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS25 at approximately 17 kDa. The expected band size for RPS25 is at 14 kDa.
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Immunofluorescent staining of human cell line EFO-21 shows localization to nucleoli, cytosol & endoplasmic reticulum.
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