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Immunohistochemical staining of human skin shows weak positivity in nucleoli in squamous epithelial cells.
Immunohistochemical staining of human skin shows weak positivity in nucleoli in squamous epithelial cells.
Immunohistochemical staining of human skin shows weak positivity in nucleoli in squamous epithelial cells.

Anti-RRP1 Antibody

HPA018166
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRRP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RRP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8568
  • Target name
    RRP1
  • Target description
    ribosomal RNA processing 1
  • Target synonyms
    D21S2056E, NNP-1, NOP52, RRP1A, ribosomal RNA processing protein 1 homolog A, Nnp1 homolog, nucleolar protein, RRP1-like protein, novel nuclear protein 1, nucleolar protein Nop52, protein NNP-1, ribosomal RNA processing 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56182
  • Protein Name
    Ribosomal RNA processing protein 1 homolog A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RRP1 using anti-RRP1 antibody (A06230-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP1 antigen affinity purified polyclonal antibody (Catalog # A06230-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP1 at approximately 53 kDa. The expected band size for RRP1 is at 53 kDa.
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