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Anti-RRP1B (N-term) Antibody

102-21813
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetRRP1B
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-RRP1B (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB27460
  • Gene ID23076
  • Target name
    RRP1B
  • Target description
    ribosomal RNA processing 1B
  • Target synonyms
    KIAA0179, NNP1L, Nnp1, PPP1R136, RRP1, ribosomal RNA processing protein 1 homolog B, RRP1-like protein B, protein phosphatase 1, regulatory subunit 136, ribosomal RNA processing 1 homolog B
  • Host
    Rabbit
  • Protein IDQ14684
  • Protein Name
    Ribosomal RNA processing protein 1 homolog B
  • Scientific Description
    Rabbit Anti-RRP1B (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRP1B using anti-RRP1B antibody (A08159-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP1B antigen affinity purified polyclonal antibody (Catalog # A08159-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP1B at approximately 95 kDa. The expected band size for RRP1B is at 84 kDa.
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