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Immunohistochemical staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.

Anti-RSBN1 Antibody

HPA049484
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRSBN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RSBN1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54665
  • Target name
    RSBN1
  • Target description
    round spermatid basic protein 1
  • Target synonyms
    KDM9; lysine-specific demethylase 9; ROSBIN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VWQ0
  • Protein Name
    Lysine-specific demethylase 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human thyroid gland shows moderate cytoplasmic and nuclear positivity in glandular cells.
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Figure 1. Western blot analysis of RSBN1 using anti-RSBN1 antibody (A15336-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSBN1 antigen affinity purified polyclonal antibody (Catalog # A15336-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSBN1 at approximately 95 kDa. The expected band size for RSBN1 is at 90 kDa.
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