Anti-RSV G protein [CB002.5]

Absolute Antibody

Anti-RSV G protein [CB002.5]

9

This antibody binds the the central conserved region of respiratory syncytial virus (RSV) G protein of strains A2 and B1. It was reported that the antibody binds HFEVFNFVPCSICSNNPTCWAIC peptide sequence in the conserved region of RSV G protein. Respirator

The binding characterization of this antibody was done using Pepscan-based ELISA. This antibody can potently neutralize both subtyes of RSV A2 and B1 in the presence of complement. However, it was incapable of neutralizing both subtype in absence of complement. The original antibody has 50% inhibitory concentration (IC50) of 0.12 nM and 0.06 nM for strains A2 and B1, respectively. This antibody also demonstrated potent neutraliztion of RSV in well-differentiated HBECs cultured at an air-liquid interface, in absence of complement, with an IC50 of 4.7 nM. The Fab version of this antibody bound G peptide with a dissociation constant of 0.52 nM and secreted form of RSV G from strain A2 (sG) with equilibrium dissociation constants (KD) of 3.3 nM (PMID: 29509814).

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

CB002.5

Mouse

IgM

Major surface glycoprotein G

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203