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Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.
Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.
Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.

Anti-RTF1 Antibody

HPA006714
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRTF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RTF1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23168
  • Target name
    RTF1
  • Target description
    RTF1 homolog, Paf1/RNA polymerase II complex component
  • Target synonyms
    GTL7, KIAA0252, RNA polymerase-associated protein RTF1 homolog, Rtf1, Paf1/RNA polymerase II complex component, homolog, ortholog of mouse gene trap locus 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92541
  • Protein Name
    RNA polymerase-associated protein RTF1 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RTF1 using anti-RTF1 antibody (A05784-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTF1 antigen affinity purified polyclonal antibody (Catalog # A05784-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTF1 at approximately 100 kDa. The expected band size for RTF1 is at 80 kDa.
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