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Anti-RTKN Antibody

144-06732
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetRTKN
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-RTKN Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6242
  • Target name
    RTKN
  • Target description
    rhotekin
  • Target synonyms
    rhotekin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BST9
  • Protein Name
    Rhotekin
  • Scientific Description
    RTKN Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: RTKN antibody at 4.66ug/ml Lane 1: A431 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 63, 62, 57 kDa Observed band size: 63 kDa
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Figure 1. Western blot analysis of RTKN using anti-RTKN antibody (A07596-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTKN antigen affinity purified polyclonal antibody (Catalog # A07596-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTKN at approximately 70 kDa. The expected band size for RTKN is at 63 kDa.
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