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Immunohistochemical staining of human lung shows moderate cytoplasmic positivity in macrophages.
Immunohistochemical staining of human lung shows moderate cytoplasmic positivity in macrophages.
Immunohistochemical staining of human lung shows moderate cytoplasmic positivity in macrophages.

Anti-RTKN2 Antibody

HPA038446
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRTKN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RTKN2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID219790
  • Target name
    RTKN2
  • Target description
    rhotekin 2
  • Target synonyms
    bA531F24.1; PH domain-containing family K member 1; pleckstrin homology domain-containing family K member 1; PLEKHK1; rhotekin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IZC4
  • Protein Name
    Rhotekin-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RTKN2 using anti-RTKN2 antibody (A12576-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTKN2 antigen affinity purified polyclonal antibody (Catalog # A12576-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTKN2 at approximately 69 kDa. The expected band size for RTKN2 is at 69 kDa.
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