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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.

Anti-RTN4IP1 Antibody

HPA036357
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRTN4IP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RTN4IP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84816
  • Target name
    RTN4IP1
  • Target description
    reticulon 4 interacting protein 1
  • Target synonyms
    NIMP, OPA10, NAD(P)H oxidoreductase RTN4IP1, mitochondrial, NOGO-interacting mitochondrial protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WWV3
  • Protein Name
    NAD(P)H oxidoreductase RTN4IP1, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RTN4IP1 using anti-RTN4IP1 antibody (A13135-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTN4IP1 antigen affinity purified polyclonal antibody (Catalog # A13135-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTN4IP1 at approximately 44 kDa. The expected band size for RTN4IP1 is at 44 kDa.
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