More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-S100A8 & S100A9 [5.5]

Anti-S100A8 & S100A9 [5.5]

Ab00815-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetS100A8
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-S100A8 & S100A9 [5.5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    mAb 5.5 has been used in immunoprecipitation and western blot experiments for the characterisation of p8,14 identified in extracts of monocytes and neutrophils. 5.5 was also used in flow cytometric analysis to show that p8,14 is not a cell surface protein, and in immunohistochemical staining to show that p8,14 is present extracellularly (Edgeworth 1991).
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA; FC; IHC; IP; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5.5
  • Gene ID6279
  • Target name
    S100A8
  • Target description
    S100 calcium binding protein A8
  • Target synonyms
    60B8AG; CAGA; calgranulin A; calprotectin L1L subunit; CFAG; CGLA; CP-10; cystic fibrosis antigen; L1Ag; leukocyte L1 complex light chain; MA387; MIF; migration inhibitory factor-related protein 8; MRP8; NIF; P8; protein S100-A8; S100-A8; urinary stone protein band A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05109
  • Protein Name
    Protein S100-A8
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    BALB/c mice were immunized using cells from acute monocytic leukemia patients. The BALB/c spleen cell suspension was fused with Sp2/0-Ag14 cells, and mAb 5.5 was isolated from the hybridoma cells either by radioimmunoassay with 125-I-labelled F(ab')2 fragments of sheep anti-mouse Ig as second layer, or by indirect immunofluorescence using rhodamine-conjugated goat anti-mouse Ig as the second layer.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
Anti-S100A8 Antibody130-10675
ApplicationsELISA
TargetS100A8
  • SizePrice
Product group Antibodies
Biomatik
S100A8 Polyclonal AntibodyCAC10380
ApplicationsELISA, ImmunoHistoChemistry
TargetS100A8
  • SizePrice
Product group Antibodies
Assay Genie
IFNA10 AntibodyCAB16880
TargetS100A8
  • SizePrice
IHC image of CSB-PA020641HA01HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
S100A8 AntibodyCSB-PA020641HA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetS100A8
  • SizePrice
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with S100A8 Polyclonal Antibody(bs-2696R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss Antibodies
S100-A8 / MRP8 Polyclonal AntibodyBS-2696R
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetS100A8
  • SizePrice
Immunohistochemistry analysis in human bone marrow and skeletal muscle tissues using AMAb91689 antibody. Corresponding S100A8 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-S100A8 AntibodyAMAB91689
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetS100A8
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-S100A8EB11513
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetS100A8
  • SizePrice
Product group Antibodies
GeneTex
S100A8 + S100A9 antibody [2Q1841]GTX14576
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetS100A8
  • SizePrice
Western blot analysis of MRP8 expression in HL-60 cell lysate.
Product group Antibodies
Boster Bio
Anti-MRP8 S100A8 Rabbit Monoclonal AntibodyM00413-1
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetS100A8
  • SizePrice
Anti-MRP8 Antibody
Product group Antibodies
Antibodies.com
Anti-MRP8 AntibodyA24952
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice