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ProductsBack/Anti-S100A9 [IID4B10]

Anti-S100A9 [IID4B10]

Ab02468-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetS100A9
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-S100A9 [IID4B10]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody was used for ELISA on recombinant S100A9 protein (CN104894074). Western blot was performed on S100A9, S100A8, S100A12 and S100A13 using this antibody (CN104894074). Immunohistochemistry was performed on human breast cancer, colon cancer, and hepatocellular carcinoma tissues using this antibody (CN104894074).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA; IHC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    IID4B10
  • Gene ID6280
  • Target name
    S100A9
  • Target description
    S100 calcium binding protein A9
  • Target synonyms
    60B8AG; CAGB; calgranulin B; calprotectin L1H subunit; CFAG; CGLB; L1AG; leukocyte L1 complex heavy chain; LIAG; MAC387; MIF; migration inhibitory factor-related protein 14; MRP14; MRP-14; NIF; P14; protein S100-A9; S100-A9
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP06702
  • Protein Name
    Protein S100-A9
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was raised by immunizing Balb/c mice with human blood cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100A9 using anti-S100A9 antibody (PB9677). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate, Lane 2: MCF-7 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody (Catalog # PB9677) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A9 at approximately 13 kDa. The expected band size for S100A9 is at 13 kDa.
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