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Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.

Anti-S100P Antibody

HPA075251
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
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Product group Antibodies
TargetS100P
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-S100P
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6286
  • Target name
    S100P
  • Target description
    S100 calcium binding protein P
  • Target synonyms
    MIG9; migration-inducing gene 9 protein; protein S100-E; protein S100-P
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25815
  • Protein Name
    Protein S100-P
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100P using anti-S100P antibody (A01963-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100P antigen affinity purified polyclonal antibody (Catalog # A01963-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100P at approximately 14 kDa. The expected band size for S100P is at 10 kDa.
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