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Immunohistochemistry analysis in human testis and endometrium tissues using Anti-SAGE1 antibody. Corresponding SAGE1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and endometrium tissues using Anti-SAGE1 antibody. Corresponding SAGE1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and endometrium tissues using Anti-SAGE1 antibody. Corresponding SAGE1 RNA-seq data are presented for the same tissues.

Anti-SAGE1 Antibody

HPA003208
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSAGE1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SAGE1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55511
  • Target name
    SAGE1
  • Target description
    sarcoma antigen 1
  • Target synonyms
    CT14, SAGE, sarcoma antigen 1, cancer/testis antigen 14, putative tumor antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NXZ1
  • Protein Name
    Sarcoma antigen 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA020670LA01HU diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of SAGE1 using anti-SAGE1 antibody (A15250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAGE1 antigen affinity purified polyclonal antibody (Catalog # A15250) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAGE1 at approximately 125 kDa. The expected band size for SAGE1 is at 99 kDa.
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