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Anti-SAP130 Antibody

144-60512
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSAP130
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SAP130 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79595
  • Target name
    SAP130
  • Target description
    Sin3A associated protein 130
  • Target synonyms
    histone deacetylase complex subunit SAP130, 130 kDa Sin3-associated polypeptide, Sin3-associated polypeptide p130, Sin3A associated protein 130kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0E3
  • Protein Name
    Histone deacetylase complex subunit SAP130
  • Scientific Description
    SAP130 Polyclonal Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of SAP130 using anti-SAP130 antibody (A07578-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP130 antigen affinity purified polyclonal antibody (Catalog # A07578-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP130 at approximately 130 kDa. The expected band size for SAP130 is at 110 kDa.
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