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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Anti-SCARB1 Antibody

HPA066285
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSCARB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SCARB1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID949
  • Target name
    SCARB1
  • Target description
    scavenger receptor class B member 1
  • Target synonyms
    CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1; CD36L1; CLA1; CLA-1; HDLQTL6; scavenger receptor class B member 1; scavenger receptor class B type III; SRB1; SR-BI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WTV0
  • Protein Name
    Scavenger receptor class B member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemistry analysis in human adrenal gland and pancreas tissues using Anti-SCARB1 antibody. Corresponding SCARB1 RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of Scavenging Receptor SR-BI/SCARB1 using anti-Scavenging Receptor SR-BI/SCARB1 antibody (A01093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scavenging Receptor SR-BI/SCARB1 antigen affinity purified polyclonal antibody (Catalog # A01093-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Scavenging Receptor SR-BI/SCARB1 at approximately 85 kDa. The expected band size for Scavenging Receptor SR-BI/SCARB1 is at 85 kDa.
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