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Immunofluorescent staining of human cell line MCF7 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line MCF7 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line MCF7 shows localization to endoplasmic reticulum.

Anti-SCD Antibody

HPA063921
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
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Product group Antibodies
TargetSCD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SCD
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6319
  • Target name
    SCD
  • Target description
    stearoyl-CoA desaturase
  • Target synonyms
    acyl-CoA desaturase; delta(9)-desaturase; FADS5; fatty acid desaturase; hSCD1; MSTP008; predicted protein of HQ0998; SCD1; SCDOS; stearoyl-CoA desaturase; stearoyl-CoA desaturase (delta-9-desaturase); stearoyl-CoA desaturase opposite strand
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00767
  • Protein Name
    Acyl-CoA desaturase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCD using anti-SCD antibody (A00588-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCD antigen affinity purified polyclonal antibody (Catalog # A00588-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCD at approximately 37,42 kDa. The expected band size for SCD is at 42 kDa.
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