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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in endothelial cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in endothelial cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in endothelial cells.

Anti-SCLT1 Antibody

HPA036560
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSCLT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SCLT1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID132320
  • Target name
    SCLT1
  • Target description
    sodium channel and clathrin linker 1
  • Target synonyms
    CAP1A; CAP-1A; sodium channel and clathrin linker 1; sodium channel-associated protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96NL6
  • Protein Name
    Sodium channel and clathrin linker 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SCLT1 using anti-SCLT1 antibody (A11417-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human COLO320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCLT1 antigen affinity purified polyclonal antibody (Catalog # A11417-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCLT1 at approximately 75 kDa. The expected band size for SCLT1 is at 81 kDa.
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