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Immunohistochemical staining of human kidney shows cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows cytoplasmic positivity in renal tubules.

Anti-SCRN2 Antibody

HPA023434
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSCRN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SCRN2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID90507
  • Target name
    SCRN2
  • Target description
    secernin 2
  • Target synonyms
    Ses2, secernin-2, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96FV2
  • Protein Name
    Secernin-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SCRN2 using anti-SCRN2 antibody (A15761-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCRN2 antigen affinity purified polyclonal antibody (Catalog # A15761-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCRN2 at approximately 42 kDa. The expected band size for SCRN2 is at 42 kDa.
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