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Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Anti-SDHB Antibody

AMAB90706
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSDHB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SDHB
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL0347
  • Conjugate
    Unconjugated
  • Gene ID6390
  • Target name
    SDHB
  • Target description
    succinate dehydrogenase complex iron sulfur subunit B
  • Target synonyms
    CWS2; IP; iron-sulfur subunit of complex II; MC2DN4; PGL4; SDH; SDH1; SDH2; SDHIP; succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP21912
  • Protein Name
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SDHB using anti-SDHB antibody (A01090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHB antigen affinity purified polyclonal antibody (Catalog # A01090) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHB at approximately 29 kDa. The expected band size for SDHB is at 29 kDa.
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