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ProductsBack/Anti-SEC24A Antibody
Western blot analysis in human cell line U-87 MG.
Western blot analysis in human cell line U-87 MG.
Western blot analysis in human cell line U-87 MG.

Anti-SEC24A Antibody

HPA056825
Atlas Antibodies
ApplicationsWestern Blot
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSEC24A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEC24A
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10802
  • Target name
    SEC24A
  • Target description
    SEC24 homolog A, COPII coat complex component
  • Target synonyms
    protein transport protein Sec24A; SEC24 family, member A; SEC24 related gene family, member A; SEC24-related protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95486
  • Protein Name
    Protein transport protein Sec24A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEC24A using anti-SEC24A antibody (A09726-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 292T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC24A antigen affinity purified polyclonal antibody (Catalog # A09726-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC24A at approximately 120 kDa. The expected band size for SEC24A is at 66 kDa.
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