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Immunohistochemical staining of human fallopian tube shows nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows nuclear and cytoplasmic positivity in glandular cells.

Anti-SECISBP2 Antibody

HPA044314
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSECISBP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SECISBP2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79048
  • Target name
    SECISBP2
  • Target description
    SECIS binding protein 2
  • Target synonyms
    SBP2; Sec insertion sequence-binding protein 2; selenocysteine insertion sequence-binding protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96T21
  • Protein Name
    Selenocysteine insertion sequence-binding protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SECISBP2 using anti-SECISBP2 antibody (A06543-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SECISBP2 antigen affinity purified polyclonal antibody (Catalog # A06543-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SECISBP2 at approximately 105 kDa. The expected band size for SECISBP2 is at 95 kDa.
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