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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Anti-SEL1L2 Antibody

HPA059121
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSEL1L2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEL1L2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80343
  • Target name
    SEL1L2
  • Target description
    SEL1L2 adaptor subunit of SYVN1 ubiquitin ligase
  • Target synonyms
    C20orf50, sel-1L2, protein sel-1 homolog 2, SEL1L2 adaptor subunit of ERAD E3 ligase, SEL1L2 adaptor subunit of ERAD E3 ubiquitin ligase, SEL1L2, ERAD E3 ligase adaptor subunit, sel-1 suppressor of lin-12-like 2, suppressor of lin-12-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5TEA6
  • Protein Name
    Protein sel-1 homolog 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEL1L2 using anti-SEL1L2 antibody (A15836-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEL1L2 antigen affinity purified polyclonal antibody (Catalog # A15836-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEL1L2 at approximately 70 kDa. The expected band size for SEL1L2 is at 78 kDa.
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