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Immunohistochemical staining of human lung shows strong cytoplasmic positivity in pneumocytes and macrophages.
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in pneumocytes and macrophages.
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in pneumocytes and macrophages.

Anti-SELENON Antibody

HPA058076
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSELENON
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SELENON
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57190
  • Target name
    SELENON
  • Target description
    selenoprotein N
  • Target synonyms
    CFTD; MDRS1; RSMD1; RSS; selenoprotein N; selenoprotein N, 1; SELN; SEPN1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZV5
  • Protein Name
    Selenoprotein N
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SELENON using anti-SELENON antibody (A31971-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SELENON antigen affinity purified polyclonal antibody (Catalog # A31971-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SELENON at approximately 66-70 kDa. The expected band size for SELENON is at 66-70 kDa.
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