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Immunohistochemical staining of human substantia nigra shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human substantia nigra shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human substantia nigra shows strong cytoplasmic positivity in neuronal cells.

Anti-SELENOT Antibody

HPA039780
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSELENOT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SELENOT Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51714
  • Target name
    SELENOT
  • Target description
    selenoprotein T
  • Target synonyms
    SELT, thioredoxin reductase-like selenoprotein T, thioredoxin reductase-like enzyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SELENOT using anti-SELENOT antibody (A31970). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: rat pancreas tissue lysates, Lane 8: mouse pancreas tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SELENOT antigen affinity purified polyclonal antibody (Catalog # A31970) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SELENOT at approximately 22 kDa. The expected band size for SELENOT is at 22 kDa.
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