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Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.

Anti-SERAC1 Antibody

HPA025716
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSERAC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SERAC1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84947
  • Target name
    SERAC1
  • Target description
    serine active site containing 1
  • Target synonyms
    protein SERAC1, serine active site-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96JX3
  • Protein Name
    Protein SERAC1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERAC1 antigen affinity purified polyclonal antibody (Catalog # A08852-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERAC1 at approximately 74, 20 kDa. The expected band size for SERAC1 is at 74 kDa.
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