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Anti-SERPINA6 Antibody

144-05803
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSERPINA6
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SERPINA6 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID866
  • Target name
    SERPINA6
  • Target description
    serpin family A member 6
  • Target synonyms
    CBG; corticosteroid-binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6; serpin A6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6; transcortin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08185
  • Protein Name
    Corticosteroid-binding globulin
  • Scientific Description
    SERPINA6 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (PA2230). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human A375 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cortisol Binding Globulin antigen affinity purified polyclonal antibody (Catalog # PA2230) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cortisol Binding Globulin at approximately 60 kDa. The expected band size for Cortisol Binding Globulin is at 53 kDa.
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