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Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.

Anti-SERPINA7 Antibody

HPA002803
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetSERPINA7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SERPINA7
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6906
  • Target name
    SERPINA7
  • Target description
    serpin family A member 7
  • Target synonyms
    mutant serpin family A member 7; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; serpin A7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; T4-binding globulin; TBG; TBGQTL; thyroxin-binding globulin; thyroxine-binding globulin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05543
  • Protein Name
    Thyroxine-binding globulin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Thyroxine Binding Globulin/SERPINA7 using anti-Thyroxine Binding Globulin/SERPINA7 antibody (A02766-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thyroxine Binding Globulin/SERPINA7 antigen affinity purified polyclonal antibody (Catalog # A02766-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thyroxine Binding Globulin/SERPINA7 at approximately 50 kDa. The expected band size for Thyroxine Binding Globulin/SERPINA7 is at 46 kDa.
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