Western blot analysis of Serum Amyloid A expression in human plasma lysate (M01362). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAA1 monoclonal antibody (Catalog # M01362) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAA1
Anti-Serum Amyloid A SAA1 Rabbit Monoclonal Antibody
M01362
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSAA1
Overview
- SupplierBoster Bio
- Product NameAnti-Serum Amyloid A Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDABAE-19
- FormulationLiquid
- Gene ID6288
- Target nameSAA1
- Target descriptionserum amyloid A1
- Target synonymsPIG4; SAA; SAA2; serum amyloid A protein; serum amyloid A-1 protein; TP53I4; tumor protein p53 inducible protein 4
- HostRabbit
- IsotypeIgG
- Protein IDP0DJI8
- Protein NameSerum amyloid A-1 protein
- Scientific DescriptionBoster Bio Anti-Serum Amyloid A SAA1 Rabbit Monoclonal Antibody catalog # M01362. Tested in WB, IHC applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203