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Western blot analysis of Serum Amyloid A expression in human plasma lysate (M01362). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAA1 monoclonal antibody (Catalog # M01362) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAA1
Western blot analysis of Serum Amyloid A expression in human plasma lysate (M01362). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAA1 monoclonal antibody (Catalog # M01362) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAA1
Western blot analysis of Serum Amyloid A expression in human plasma lysate (M01362). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAA1 monoclonal antibody (Catalog # M01362) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAA1

Anti-Serum Amyloid A SAA1 Rabbit Monoclonal Antibody

Research Use Only
M01362
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSAA1
Price on request
100 ul
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Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Serum Amyloid A Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ABAE-19
  • Formulation
    Liquid
  • Gene ID6288
  • Target name
    SAA1
  • Target description
    serum amyloid A1
  • Target synonyms
    PIG4; SAA; SAA2; serum amyloid A protein; serum amyloid A-1 protein; TP53I4; tumor protein p53 inducible protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP0DJI8
  • Protein Name
    Serum amyloid A-1 protein
  • Scientific Description
    Boster Bio Anti-Serum Amyloid A SAA1 Rabbit Monoclonal Antibody catalog # M01362. Tested in WB, IHC applications. This antibody reacts with Human.
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203