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Immunofluorescent staining of human cell line RT4 shows localization to intermediate filaments.
Immunofluorescent staining of human cell line RT4 shows localization to intermediate filaments.
Immunofluorescent staining of human cell line RT4 shows localization to intermediate filaments.

Anti-SESTD1 Antibody

HPA077408
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSESTD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SESTD1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID91404
  • Target name
    SESTD1
  • Target description
    SEC14 and spectrin domain containing 1
  • Target synonyms
    Solo, SEC14 domain and spectrin repeat-containing protein 1, SEC14 and spectrin domains 1, huntingtin-interacting protein-like protein, protein Solo
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86VW0
  • Protein Name
    SEC14 domain and spectrin repeat-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Solo/SESTD1 using anti-Solo/SESTD1 antibody (A10698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Solo/SESTD1 antigen affinity purified polyclonal antibody (Catalog # A10698-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Solo/SESTD1 at approximately 79 kDa. The expected band size for Solo/SESTD1 is at 79 kDa.
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