Anti-Severe Fever and Thrombocytopenic Syndrome Virus [MAb4-5]

Absolute Antibody

Anti-Severe Fever and Thrombocytopenic Syndrome Virus [MAb4-5]

9

The antibody is specific for SFTSV. It binds to a linear epitope in the ectodomain of glycoprotein Gn. It does not cross react with RVFV.

The antibody showed neutralizing activity against SFTSV infection in Vero cells (Guo et al. 2013; PMID: 23863504). To verify neutralizing activity, full-length human IgG1 antibody was constructed. The recombinant antibodies were expressed by 293T mammalian cells and the concentration required to obtain 50% neutralization of 100 TCID50 SFTSV in vitro was 44.2 microg/mL. The interaction between Gn and the antibody was further demonstrated by surface plasmon resonance (SPR) assays with a dissociation constant (Kd) of 25.9 nM. The crystal structure of SFTSV Gn head domain in complex with the Fab fragment of the antibody was determined. The antibody could recognize the denatured SFTSV Gn head by WB (Wu et al., 2017; PMID: 28827346).

Western Blot, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

MAb4-5

Human

IgG1

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203