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Immunohistochemical staining of human kidney shows distinct positivity in glomeruli, renal tubules showed nucleolar positivity.
Immunohistochemical staining of human kidney shows distinct positivity in glomeruli, renal tubules showed nucleolar positivity.
Immunohistochemical staining of human kidney shows distinct positivity in glomeruli, renal tubules showed nucleolar positivity.

Anti-SFR1 Antibody

HPA039617
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSFR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SFR1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID119392
  • Target name
    SFR1
  • Target description
    SWI5 dependent homologous recombination repair protein 1
  • Target synonyms
    C10orf78, MEI5, MEIR5, bA373N18.1, swi5-dependent recombination DNA repair protein 1 homolog, MEI5 recombination repair protein homolog, SWI5-dependent recombination repair 1, SWI5-dependent recombination repair protein 1, meiosis protein 5 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86XK3
  • Protein Name
    Swi5-dependent recombination DNA repair protein 1 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SFR1 using anti-SFR1 antibody (A07683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFR1 antigen affinity purified polyclonal antibody (Catalog # A07683-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFR1 at approximately 22 kDa. The expected band size for SFR1 is at 28 kDa.
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