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Immunohistochemical staining of human lung shows strong cytoplasmic and membranous positivity in pneumocytes.
Immunohistochemical staining of human lung shows strong cytoplasmic and membranous positivity in pneumocytes.
Immunohistochemical staining of human lung shows strong cytoplasmic and membranous positivity in pneumocytes.

Anti-SFTPA1 Antibody

HPA045752
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSFTPA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SFTPA1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID653509
  • Target name
    SFTPA1
  • Target description
    surfactant protein A1
  • Target synonyms
    COLEC4, ILD1, PSAP, PSP-A, PSPA, SFTP1, SFTPA1B, SP-A, SP-A1, SP-A1 beta, SP-A1 delta, SP-A1 epsilon, SP-A1 gamma, SPA, SPA1, pulmonary surfactant-associated protein A1, 35 kDa pulmonary surfactant-associated protein, alveolar proteinosis protein, collectin-4, surfactant protein A1B, surfactant, pulmonary-associated protein A1A, surfactant, pulmonary-associated protein A1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWL2
  • Protein Name
    Pulmonary surfactant-associated protein A1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1/2 antigen affinity purified polyclonal antibody (Catalog # PB9390) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1/2 at approximately 26-38 kDa. The expected band size for SFTPA1/2 is at 26 kDa.
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