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Anti-SFXN3 Antibody

144-62282
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSFXN3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SFXN3 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID81855
  • Target name
    SFXN3
  • Target description
    sideroflexin 3
  • Target synonyms
    BA108L7.2, SFX3, SLC56A3, sideroflexin-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BWM7
  • Protein Name
    Sideroflexin-3
  • Scientific Description
    SFXN3 pAb
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87 MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFXN3 antigen affinity purified polyclonal antibody (Catalog # A14648-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFXN3 at approximately 36 kDa. The expected band size for SFXN3 is at 36 kDa.
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