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Immunohistochemical staining of human duodenum shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows cytoplasmic and membranous positivity in glandular cells.

Anti-SFXN5 Antibody

HPA015473
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSFXN5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SFXN5
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID94097
  • Target name
    SFXN5
  • Target description
    sideroflexin 5
  • Target synonyms
    BBG-TCC, SLC56A5, sideroflexin-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TD22
  • Protein Name
    Sideroflexin-5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFXN5 antigen affinity purified polyclonal antibody (Catalog # A15698-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFXN5 at approximately 37 kDa. The expected band size for SFXN5 is at 37 kDa.
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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & mitochondria.
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