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Anti-SH3GL2 Antibody

144-05701
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetSH3GL2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SH3GL2 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6456
  • Target name
    SH3GL2
  • Target description
    SH3 domain containing GRB2 like 2, endophilin A1
  • Target synonyms
    bA335L15.1 (SH3-domain GRB2-like 2); CNSA2; EEN-B1; Endophilin A1 BAR domain; endophilin-1; endophilin-A1; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3D2A; SH3-domain GRB2-like 2; SH3P4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99962
  • Protein Name
    Endophilin-A1
  • Scientific Description
    SH3GL2 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SH3GL2 using anti-SH3GL2 antibody (A05430-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3GL2 antigen affinity purified polyclonal antibody (Catalog # A05430-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3GL2 at approximately 41 kDa. The expected band size for SH3GL2 is at 41 kDa.
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