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Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.

Anti-SHC1 Antibody

HPA001844
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSHC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SHC1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6464
  • Target name
    SHC1
  • Target description
    SHC adaptor protein 1
  • Target synonyms
    SHC, SHCA, SHC-transforming protein 1, SH2 domain protein C1, SHC (Src homology 2 domain containing) transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29353
  • Protein Name
    SHC-transforming protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SHC using anti-SHC antibody (PB9391). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate, Lane 2: human Hela whole cell lysate, Lane 3: human HepG2 whole cell lysate, Lane 4: human Jurkat whole cell lysate, Lane 5: human K562 whole cell lysate, Lane 6: human THP-1 whole cell lysate, Lane 7: rat C6 whole cell lysate, Lane 8: mouse thymus tissue lysate, Lane 9: mouse RAW246.7 whole cell lysate, Lane 10: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody (Catalog # PB9391) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SHC at approximately 46, 52, 66KD. The expected band size for SHC are at 46, 52, 66KD.
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